Cell Line
MCF-7; MCF 7; MCF.7; MCF7; Michigan Cancer Foundation-7; ssMCF-7; ssMCF7; MCF7/WT; IBMF-7; MCF7-CTRL
Organism
human
Tissue/Cell type
adenocarcinoma, breast cancer, mammary gland, epithelial
Genetically modified (GenTSV)
Risk group (BioStoffV)
1
Risk assessment
According to ZKBS (https://zag.bvl.bund.de/zelllinien/detail.jsf?id=102&dswid=2452&dsrid=273)
Storage location of aliquots in the Biolab (just click Bearbeiten in the right corner of the header to add or change information in the table and use the menue in the left header to e.g. add a row)
| source | freezing date | passage no. | cell count per vial | cell culture medium incl. supplements | no. of aliquots | belongs to (full name) | rack/box in N2 tank or -80°° freezer and location (room, address) | comments |
|---|---|---|---|---|---|---|---|---|
| DSMZ | 27.03.2019 | px+9 | 16 | RPMI, 10% FBS, 1% PS | 13 | Katharina Achazi (Scarlett Manzke) | 03/04, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| DSMZ | 14.01.2017 | 1,80E+05 | 6 | Jie Chen, AG Ma (Katharina Achazi) | 01/07, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||
| DSMZ | 09.04.2017 | 2 | Jie Chen, AG Ma (Katharina Achazi) | 03/08, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||||
| Mivenion | 17.12.2015 | p54 | DMEM | 1 | Katharina Achazi (Paul Hillmann) | 03/07, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||
| Charité - University Medicine Berlin (ATCC) | 05.03.2016 | P 3 (since me) | 3500000 | DMEM 10% FBS 1% P/S | 7 | Yan Li, AG Ma (Katharina Achazi) | 03/08, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| DSMZ | 03.03.2014 | p5 | 5000000 | RPMI | 5 | Katharina Achazi | 11/05, N2 tank,(SupraFab, Altensteinstraße 23a, 114.1) | |
| DSMZ | 31.10.2017 | px+7 | 5 | Katharina Achazi (Jing) | 11/05, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||
| DSMZ | 18.12.2015 | Stefanie Wedepohl | 11/08, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | Does not exist anymore? NCIIADR, dox resistent | ||||
| Ulrich Brinkmann (Roche) (DSMZ) | 09.04.2014 | RPMI 10% FBS, 1% P/S | 2 | Stefanie Wedepohl | 05/04, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |||
| DSMZ | 21.11.2016 | p3 | RPMI | 7 | Katharina Achazi (Zhaoxu/Wei) | 12/05, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||
| DSMZ | 28.03.2014 | p7 | RPMI | 9 | Katharina Achazi (CK) | 12/06, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||
| DSMZ | 19.10.2016 | 2 | Yan Li, AG Ma (Katharina Achazi) | 03/10, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | ||||
| Mivenion | 16.07.2020 | p59 | 1000000 | DMEM 10% FBS 1% P/S | 5 | Katharina Achazi/Elisa Quaas | 03/07, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| Mivenion | 15.12.2020 | p77 | 1000000 | DMEM 10% FBS 1% P/S | 8 | Elisa Quaas | 03/07, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| Mivenion | 18.03.2021 | p96 | 1000000 | DMEM 10% FBS 1% P/S | 3 | Elisa Quaas | 04/06, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| Mivenion | 3103.2022 | p101 | 1000000 | DMEM 10% FBS 1% P/S | 10 | Elisa Quaas | 04/07, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| Mivenion | 19.07.2022 | p110 | 1000000 | DMEM 10% FBS 1% P/S | 7 | Elisa Quaas | 04/07, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | tested mycoplasm free |
| DSMZ | 16.06.2021 | px+2 | 500000 | RPMI 10% FBS, 1% P/S | 15 | Peng Tang | 02/07, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| DSMZ | 08.08.2022 | px+5 | 1000000 | RPMI 10% FBS, 1% P/S | 12 | Peng Tang | 03/07, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) | |
| Mivenion | 17.11.2023 | P111 | 3000000 | DMEM 10% FBS 1% P/S | 10 | Kunpeng Liu | 05/07, N2 tank, (SupraFab, Altensteinstraße 23a, 114.1) |
Background
Cultivation and freezing protocols
Data sheets, further information
https://web.expasy.org/cellosaurus/CVCL_0031
https://www.dsmz.de/collection/catalogue/details/culture/ACC-115
